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Exam 16: Genomics

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Question 1

Multiple Choice

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A serious problem encountered with artificial chromosomes like YACs is

A) recombination within the host cell.
B) the difficulty of introducing them into cells.
C) the large size of the molecules, making them difficult to construct.
D) A and B
E) none of the above.

F) All of the above
G) A) and B)

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A

Question 2

True/False

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Cloning vectors are used to introduce novel DNA into cells.

A) True
B) False

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Question 3

Multiple Choice

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Which restriction enzyme leaves blunt ends?

It is sometimes not easy to locate or identify genes based on cDNA. How do cDNA and DNA with respect to a given gene differ to create this problem?

A DNA copy of a messenger RNA molecule is called complementary DNA or cDNA.

Calculate the number of copies produced from a single starting molecule after 10 rounds of PCR.

In DNA electrophoresis, fragment separation is based on

PCR is a genetic technique developed in the 1950s.

The first available drug manufactured with recombinant DNA technology was insulin, marked under the name Humulin.

A DNA copy of an mRNA molecule is called

Cloning vectors generally possess unique restriction sites and dominant selectable markers.

Successful insertion of a DNA fragment into the polylinker region of pUC19 is detected by

A(n) ________ is basically a photograph using X-ray film, used in such genetics applications as identifying the position of radiolabeled molecules on a gel.

Polymerase chain reaction has revolutionized genetics because

PCR has revolutionized the criminal justice system through DNA fingerprinting. For a given fragment of DNA, showing that a suspect's fingerprint differs from that found at a crime scene immediately excludes him or her from suspicion, yet finding a match does not mean that guilt can be automatically concluded. Why?

The recognition sequences for many restriction enzymes are

Many bacteria are naturally capable of taking up endogenous fragments of DNA in their environment, a phenomenon termed

PCR has opened the door to analysis of ancient DNA-DNA taken from long-dead organisms. The most useful DNA fragments in such cases typically come from mitochondrial DNA. Why do you think that is so?

Say you were using random PCR primers to amplify fragments of DNA. Would you expect to get more or less amplification products if you set the PCR machine at a relatively higher or lower annealing temperature, respectively, and why?

A circular DNA molecule with two restriction enzyme cut sites will yield three fragments upon digestion.