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An agent used to reduce the number of bacteria on a toilet would most accurately be called a(n)


A) disinfectant.
B) aseptic.
C) fungicide.
D) antiseptic.
E) virucide.

F) B) and D)
G) A) and B)

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All of the following factors contribute to hospital-acquired infections EXCEPT


A) some bacteria metabolize disinfectants.
B) bacteria may be present in commercial products such as mouthwash.
C) gram-negative bacteria are often resistant to disinfectants.
D) invasive procedures can provide a portal of entry for bacteria.
E) None of the answers is correct; all of these factors may contribute to hospital -acquired infection.

F) C) and D)
G) B) and D)

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The pH of the medium has no effect on the activity of the disinfectant being applied.

A) True
B) False

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Which of the following best describes the pattern of microbial death?


A) The cells in a population die at a constant rate.
B) Not all of the cells in a culture are killed.
C) The pattern varies depending on the species.
D) All the cells in a culture die at once.
E) The pattern varies depending on the antimicrobial agent.

F) C) and D)
G) B) and C)

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Application of heat to living cells can result in all of the following EXCEPT


A) decreased thermal death time.
B) alteration of membrane permeability.
C) damage to nucleic acids.
D) breaking of hydrogen bonds.
E) denaturation of enzymes.

F) A) and E)
G) C) and D)

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Bone and tendons for transplant are decontaminated by


A) peroxygens.
B) ethylene oxide.
C) supercritical fluids.
D) plasma sterilization.
E) glutaraldehyde.

F) A) and C)
G) B) and E)

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C

All of the following are methods of food preservation EXCEPT


A) ionizing radiation.
B) high pressure.
C) osmotic pressure.
D) microwaves.
E) desiccation.

F) All of the above
G) B) and D)

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Which of the following chemical agents is used for sterilization?


A) alcohol
B) ethylene oxide
C) phenolics
D) chlorine
E) soap

F) C) and E)
G) All of the above

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B

Which of the following regarding antimicrobial control agents is FALSE?


A) Some agents kill by denaturing microbial cell proteins.
B) Alcohols effectively inactivate nonenveloped viruses by attacking lipids.
C) Contaminating organic debris such as blood or sputum decrease effectiveness.
D) Silver is used for treating antibiotic-resistant bacteria.
E) Some agents affect microbial cell membranes by dissolving lipids.

F) A) and C)
G) A) and E)

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Figure 7.1 Figure 7.1    A suspension of 106 Bacillus cereus endospores was put in a hot-air oven at 170°C. Plate counts were used to determine the number of endospores surviving at the time intervals shown. -In Figure 7.1, the thermal death point for this culture is A)  15 minutes. B)  30 minutes. C)  50°C. D)  170°C. E)  The answer cannot be determined based on the information provided A suspension of 106 Bacillus cereus endospores was put in a hot-air oven at 170°C. Plate counts were used to determine the number of endospores surviving at the time intervals shown. -In Figure 7.1, the thermal death point for this culture is


A) 15 minutes.
B) 30 minutes.
C) 50°C.
D) 170°C.
E) The answer cannot be determined based on the information provided

F) B) and E)
G) A) and D)

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The antimicrobial activity of chlorine is due to which of the following?


A) the formation of a hypochlorite ion
B) the formation of ozone
C) the formation of hypochlorous acid
D) the formation of hydrochloric acid
E) disruption of the plasma membrane

F) None of the above
G) A) and B)

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Which of the following does NOT achieve sterilization?


A) autoclave
B) pasteurization
C) supercritical fluids
D) dry heat
E) ethylene oxide

F) A) and B)
G) A) and C)

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Any process that destroys the non-spore forming contaminants on inanimate objects is sterilization.

A) True
B) False

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False

Which of the following pairs is mismatched?


A) organic acids - food preservation
B) Ag - wound dressings
C) alcohols - open wounds
D) CuSO₂ - algicide
E) H₂O₂ - open wounds

F) None of the above
G) B) and D)

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Which of the following substances is used for surgical hand scrubs?


A) phenol
B) glutaraldehyde
C) chlorine bleach
D) soap
E) chlorhexidine

F) B) and E)
G) C) and D)

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Table 7.1 A disk-diffusion test using Staphylococcus gave the following results:  Disinfectant  Zone of inhibition (mm)   A 0 B 2.5 C 10 D 5\begin{array} { c c } \text { Disinfectant } & \text { Zone of inhibition (mm) } \\\hline \text { A } & 0 \\\text { B } & 2.5 \\\text { C } & 10 \\\text { D } & 5\end{array} -In Table 7.1, which compound was the most effective against E. coli?


A) A
B) B
C) C
D) D
E) The answer cannot be determined based on the information provided.

F) A) and B)
G) None of the above

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Which of the following methods is used to preserve food by slowing the metabolic processes of foodborne microbes?


A) pasteurization
B) lyophilization
C) ionizing radiation
D) nonionizing radiation
E) freezing

F) None of the above
G) A) and D)

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Which of the following pairs is mismatched?


A) ionizing radiation - hydroxyl radicals
B) ultraviolet radiation - desiccation
C) supercritical fluids - CO₂
D) plasma sterilization - free radicals
E) ozone - takes electrons from substances

F) A) and D)
G) D) and E)

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The thermal death time is the time needed to kill all the bacteria in a particular culture at a certain temperature.

A) True
B) False

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Some antimicrobial chemicals are considered to be disinfectants and antiseptics.

A) True
B) False

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