Question 1

__________ are used in the detection of DNA by FISH.

Accepted Answer

A)   Restriction fragments
B)   Reverse transcriptase and DNA polymerase
C)   Synthetic DNAs and fluorescent tags
D)   Compressed air and gold beads
E)   DNA polymerase and DNA ligaseF) B) and E)
G) C) and E)

Question 2

Which of the following is essential in PCR?

Accepted Answer

A)   DNA polymerase
B)   antisense RNAs
C)   reverse transcriptase
D)   DNA primers
E)   both DNA primers and DNA polymeraseF) A) and B)
G) A) and C)

Question 3

The process known as PCR involves the use of

Accepted Answer

A)   DNA polymerase.
B)   RNA polymerase.
C)   reverse transcriptase.
D)   DNA ligase.
E)   restriction enzymes.F) D) and E)
G) B) and E)

Question 4

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Accepted Answer

A)   the modification of crop plants
B)   screening of humans for genes that predispose them to disease
C)   unforeseen impact on the environment
D)   the modification of animals to produce pharmaceuticals for humans
E)   answering basic research questionsF) B) and C)
G) B) and D)

Question 5

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Accepted Answer

A)   Deinococcus radiodurans
B)   Bacillus thuringiensis
C)   Pseudomonas
D)   Salmonella
E)   Escherichia coliF) A) and C)
G) B) and E)

Question 6

Compare and contrast the two major types of restriction enzymes.

Accepted Answer

All restriction enzymes recognize specif...

Question 7

The process of locating genes within the nucleic acid of an organism is called __________ mapping.

Accepted Answer

The answer of The process of locating genes within the...

Question 8

Synthesis of cDNA __________ requires the use of

Accepted Answer

A)   DNA ligase.
B)   agarose.
C)   reverse transcriptase.
D)   restriction enzymes.
E)   fluorescent synthetic nucleotides.F) A) and E)
G) A) and D)

Question 9

Injecting DNA into cells can be accomplished using

Accepted Answer

A)   nitrocellulose membranes.
B)   silicon chips and nucleic acids.
C)   compressed air and gold beads.
D)   micropipettes.
E)   micropipettes, or compressed air and gold beads.F) D) and E)
G) A) and B)

Question 10

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Accepted Answer

A) True 
 B)False

Question 11

Nitrocellulose membranes used in Southern blotting are more delicate than agarose gels.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following devices is used for PCR?

Accepted Answer

A)   an electrophoresis chamber
B)   a gene gun
C)   a DNA sequencer
D)   a thermocycler
E)   a nucleic acid synthesis machineF) D) and E)
G) C) and D)

Question 13

A northern blot differs from a Southern blot in

Accepted Answer

A)   the type of probe used.
B)   the presence or absence of a nitrocellulose membrane.
C)   the size of the genetic sequences involved.
D)   the number of genetic sequences detected.
E)   the type of nucleic acid being isolated.F) A) and E)
G) None of the above

Question 14

Beta-carotene, the biochemical precursor to vitamin A, can be added to rice by using __________ DNA technology, thereby increasing its nutritional value.

Accepted Answer

The answer of Beta-carotene, the biochemical precursor to vitamin A,...

Question 15

Which of the following microbes produces a protein that kills a variety of insect pests?

Accepted Answer

A)   Pseudomonas
B)   Haemophilus influenzae
C)   Bacillus thuringiensis
D)   Thermus aquaticus
E)   Plasmodium falciparumF) C) and E)
G) B) and C)

Question 16

Nucleic acid molecules used to deliver new genes to cells are called __________.

Accepted Answer

The answer of Nucleic acid molecules used to deliver new...

Question 17

Which of the following items is NOT a part of the name of a restriction enzyme?

Accepted Answer

A)   the Gram reaction of the source bacterium
B)   the specific epithet of the source bacterium
C)   Roman numerals to indicate its order of discovery
D)   the strain of the source bacterium
E)   the genus of the source bacteriumF) A) and E)
G) B) and D)

Question 18

Vectors usually contain genetic __________ such as antibiotic genes or fluorescent tags.

Accepted Answer

The answer of Vectors usually contain genetic __________ such as...

Question 19

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a patient's blood?

Accepted Answer

A)   Southern blotting
B)   creation of a gene library
C)   electroporation
D)   genome mapping
E)   xenotransplantationF) A) and D)
G) None of the above

Question 20

The use of microbes to make practical products such as vaccines or hormones is called __________.

Accepted Answer

The answer of The use of microbes to make practical...

Exam 8: Recombinant Dna Technology

Nucleic acid molecules used to deliver new genes to cells are called __________.

Correct AnswerverifiedShow Answer

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a patient's blood?

Correct AnswerverifiedShow Answer

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Correct AnswerverifiedShow Answer

Which of the following microbes produces a protein that kills a variety of insect pests?

Correct AnswerverifiedShow Answer

Compare and contrast the two major types of restriction enzymes.

Correct AnswerverifiedAll restriction enzymes recognize specif...View AnswerShow Answer

A northern blot differs from a Southern blot in

Correct AnswerverifiedShow Answer

__________ are used in the detection of DNA by FISH.

Correct AnswerverifiedShow Answer

Which of the following devices is used for PCR?

Correct AnswerverifiedShow Answer

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Correct AnswerverifiedShow Answer

Which of the following is essential in PCR?

Correct AnswerverifiedShow Answer

The use of microbes to make practical products such as vaccines or hormones is called __________.

Correct AnswerverifiedShow Answer

Which of the following items is NOT a part of the name of a restriction enzyme?

Correct AnswerverifiedShow Answer

The process of locating genes within the nucleic acid of an organism is called __________ mapping.

Correct AnswerverifiedShow Answer

Nitrocellulose membranes used in Southern blotting are more delicate than agarose gels.

Correct AnswerverifiedShow Answer

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Correct AnswerverifiedShow Answer

The process known as PCR involves the use of

Correct AnswerverifiedShow Answer

Beta-carotene, the biochemical precursor to vitamin A, can be added to rice by using __________ DNA technology, thereby increasing its nutritional value.

Correct AnswerverifiedShow Answer

Injecting DNA into cells can be accomplished using

Correct AnswerverifiedShow Answer

Vectors usually contain genetic __________ such as antibiotic genes or fluorescent tags.

Correct AnswerverifiedShow Answer

Synthesis of cDNA __________ requires the use of

Correct AnswerverifiedShow Answer

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All restriction enzymes recognize specif...
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